资源类型

期刊论文 127

会议视频 1

年份

2023 15

2022 24

2021 8

2020 19

2019 4

2018 7

2017 11

2016 3

2015 4

2014 8

2013 4

2012 7

2011 3

2010 2

2009 1

2008 1

2007 1

2003 1

2001 2

展开 ︾

关键词

DX桩 3

岩爆 2

数值分析 2

水产养殖 2

沉降 2

海洋鱼类增殖和养殖 2

CCS 1

CO2-ECBM 1

Inconel 718合金 1

Laves相 1

X 射线成像 1

三维扫描振镜 1

三维测量 1

中草药 1

互养菌（Syntrophus） 1

互营杆菌（Syntrophorhabdaceae） 1

人工繁殖 1

代谢组 1

优化设计 1

展开 ︾

检索范围：

排序： 展示方式：

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

《环境科学与工程前沿（英文）》 2011年 第5卷 第2期   页码 277-282 doi: 10.1007/s11783-010-0226-x

摘要： Sequencing batch reactor (SBR) for enhanced biological phosphorus removal (EBPR) processes was used to investigate the impact of the temperature shock on the competition between phosphorus-accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in start-up stage. During the 34 days operation, SBR was set with temperature variation(0–5 d, 22±1°C; 6–13 d, 29±1°C; 14–34 d, 14±1°C). PAOs and GAOs were analyzed by fluorescent in situ hybridization (FISH), and intracellular polyphosphate granules were stained by Neisser-stain. The results showed that the influence of temperature shock on PAOs’ abundance was more serious than that on GAOs in the enriching process. Under sudden and substantially temperature variation, from 22±1°C to 29±1°C and then to 14±1°C, the domination of PAOs was deteriorated. After temperature shock, PAOs’ competitive advantages at low temperature that concluded in other study did not appear in our study. As mesophilic, GAOs (indicated by and ) were more temperature adaptive and better grew and took the domination at 14±1°C in the end. In the competition process, organisms of tetrad forming organisms (TFOs)-like shape which were considered as typical GAOs, were observed. With the evidence of poly-P granules containing by Neisser-straining and result of FISH, these organisms of TFOs-like shape were better to be assumed as adaption state or a special self-protecting shape of PAOs.

关键词： fluorescent in situ hybridization (FISH)     tetrad forming organisms (TFOs)     temperature variation     enhanced biological phosphorus removal (EBPR)    

HTML PDF 收藏

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

《医学前沿（英文）》 2023年 第17卷 第3期   页码 493-502 doi: 10.1007/s11684-022-0946-x

摘要： Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

关键词： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry     variant allele frequency     intratumoral heterogeneity     targeted therapy    

HTML PDF 收藏

Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products in fish

Yong YU,Laosheng WU

《环境科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 475-481 doi: 10.1007/s11783-014-0640-6

摘要： Endocrine disrupting compounds (EDCs), pharmaceuticals and personal care products (PPCPs) have attracted much attention due to widespread contamination in aquatic environment. In this study, we determined 13 EDCs and PPCPs in fish blood, bile and muscle by using gas chromatography-mass spectrometry (GC-MS). The limits of quantitation (LOQ) were in the ranges of 0.23–2.54, 0.22–2.36 ng·mL , and 0.24–2.57 ng·g dry weight (dw) for fish blood, bile and muscle, respectively. Recoveries of target compounds spiked into sample matrices and passed through the entire analytical procedure ranged from 65% to 95%, from 60% to 92% and from 62% to 91% for blood, bile and muscle, respectively. The methods were applied to the analysis of fish from a lake in California. Target compounds were relatively low in bile, and only bisphenol A (BPA) and diclofenac were measurable near the LOQ. Seven of 13 compounds were detected in blood, with total concentrations up to 39 ng·mL . Only BPA was frequently found in muscle, with mean concentration of 7.26 ng·g dw. The estimated daily intake of BPA through fish consumption for U.S. resident was significantly lower than the tolerable daily intake recommended by the European Food Safety Authority. This study showed that the exposure to the bisphenol A from fish diet is unlikely to pose a health risk.

关键词： endocrine disrupting compounds (EDCs)     pharmaceuticals and personal care products (PPCPs)     fish     bisphenol A (BPA)     risk assessment    

HTML PDF 收藏

Information gathering and processing with fluorescent molecules

Brian DALY,Jue LING,A. Prasanna de SILVA

《化学科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 240-251 doi: 10.1007/s11705-014-1432-z

摘要： Molecular information gathering and processing — a young field of applied chemistry — is undergoing good growth. The progress is occurring both in terms of conceptual development and in terms of the strengthening of older concepts with new examples. This review critically surveys these two broad avenues. We consider some cases where molecules emulate one of the building blocks of electronic logic gates. We then examine molecular emulation of various Boolean logic gates carrying one, two or three inputs. Some single-input gates are popular information gathering devices. Special systems, such as ‘lab-on-a-molecule’ and molecular keypad locks, also receive attention. A situation deviating from the Boolean blueprint is also discussed. Some pointers are offered for maintaining the upward curve of the field.

关键词： molecular logic     molecular computation     molecular sensor     fluorescent molecular device     fluorescent sensor    

HTML PDF 收藏

基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究 Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

《工程（英文）》 2022年 第9卷 第2期   页码 61-66 doi: 10.1016/j.eng.2021.05.012

摘要：

利用荧光原位杂交（FISH）和克隆文库构建技术，研究了厌氧污泥床反应器处理含酚废水时所收获的颗粒的微生物群落结构。对厌氧颗粒薄片的FISH 分析显示细菌和古菌呈随机分布。然而，互营杆菌、隐杆厌氧菌和互养菌分布明确。在颗粒细胞的外层发现了隐杆厌氧菌和互养菌，两者关系密切，而互营杆菌则位于颗粒细胞的深层。

关键词： 隐杆厌氧菌（Cryptanaerobacter）     荧光原位杂交     苯酚厌氧降解     互营杆菌（Syntrophorhabdaceae）     互养菌（Syntrophus）     升流式厌氧污泥床（UASB）颗粒污泥    

HTML PDF 收藏

海洋鱼类资源的可持续利用和海洋鱼类科学技术的研究方向

林浩然

《中国工程科学》 2003年 第5卷 第3期   页码 27-30

摘要：

积极实施海洋鱼类的增殖与养殖是海洋鱼类资源合理开发和可持续利用的基本措施和有效途径。因此，建立和提高海洋鱼类增殖与养殖的新技术，是近年来海洋生物技术的重要研究领域之一。海洋鱼类的生殖内分泌生理学、生长与发育生物学、免疫学、分子生物学等是海洋鱼类增殖与养殖的理论基础；系统深入地开展这些学科的基础研究和应用基础研究，成为当前海洋鱼类科学技术的前沿和主要发展方向。

关键词： 海洋鱼类资源     可持续利用     海洋鱼类增殖和养殖     海洋鱼类科学技术     海洋生物技术    

HTML PDF 收藏

Recent advances of small-molecule fluorescent probes for detecting biological hydrogen sulfide

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 34-63 doi: 10.1007/s11705-021-2050-1

摘要： H2S is well-known as a colorless, acidic gas, with a notoriously rotten-egg smell. It was recently revealed that H2S is also an endogenous signaling molecule that has important biological functions, however, most of its physiology and pathology remains elusive. Therefore, the enthusiasm for H2S research remains. Fluorescence imaging technology is an important tool for H2S biology research. The development of fluorescence imaging technology has realized the study of H2S in subcellular organelles, facilitated by the development of fluorescent probes. The probes reviewed in this paper were categorized according to their chemical mechanism of sensing and were divided into three groups: H2S reducibility-based probes, H2S nucleophilicity-based probes, and metal sulfide precipitation-based probes. The structure of the probes, their sensing mechanism, and imaging results have been discussed in detail. Moreover, we also introduced some probes for hydrogen polysulfides.

关键词： hydrogen sulfide     fluorescent probe     reducibility     nucleophilicity     copper sulfide precipitate     hydrogen polysulfides    

HTML PDF 收藏

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 121-122 doi: 10.15302/J-FASE-2017142

HTML PDF 收藏

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 48-58 doi: 10.15302/J-FASE-2016114

摘要： Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

关键词： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

HTML PDF 收藏

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

《环境科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 616-624 doi: 10.1007/s11783-013-0505-4

摘要： The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifying phosphorus (P) removal performance and N O production was examined using a denitrifying enhanced biologic phosphorus removal (EBPR) sludge acclimatized with mixed acetate (HAc) and propionate (Pro) (in the molar ratio 3∶1) as carbon sources. The results showed that when the AnRT was prolonged from 90 to 150 min, the anaerobic polyhydroxyalkanoate (PHA) synthesis was decreased by 15.3%. Moreover, the ineffective PHA consumption occurred in anaerobic phases and contributed to an increased accumulation and higher free nitrous acid (FNA) concentrations (≥0.001–0.0011 mg HNO -N/L) in the subsequent anoxic phases, causing a severe inhibition on anoxic P-uptake and denitrification. Accordingly, the total nitrogen (TN) and total phosphorus (TP) removal efficiencies dropped by approximately 6.3% and 85.5%, respectively; and the ratio of anoxic N O-N production to TN removal increased by approximately 3.8%. The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated by (62.0% (SE = 1.5%)). In conclusion, the short-term excessive anaerobic reaction time negatively impacted denitrifying P removal performance and stimulated more N O production, and its effect on P removal was more obvious than that on nitrogen removal.

关键词： Denitrifying phosphorus removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescence in-situ hybridization    

HTML PDF 收藏

Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 106-115 doi: 10.15302/J-FASE-2017133

摘要： A series of dehydroabietic acid-based diarylamines have been synthesized in order to investigate their fluorescent properties, photostability, cell toxicity and fluorescence imaging. The geometries as well as their molecular properties were optimized at the B3LYP/6-31G* level using Gaussian 03. The results indicate that molecular geometry, HOMO and LUMO energies, and energy gaps are important to predict absorption and fluorescent properties. Five of the compounds can be effectively taken up by human cervical carcinoma, human hepatocellular carcinoma SMMC-7721, human gastric cancer SGC-7901 and human lung adenocarcinoma A549 cells and strong blue fluorescent signals are detected in these cells. These compounds are potential candidates for fluorescent probes in biological diagnosis.

关键词： dehydroabietic acid-based diarylamine     DFT study     fluorescent probe     in vitro imaging    

HTML PDF 收藏

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

《化学科学与工程前沿（英文）》 2023年 第17卷 第1期   页码 24-33 doi: 10.1007/s11705-022-2171-1

摘要： Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 μmol·L–1, significantly lower than that of the threshold value of 0.312 μmol·L–1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 μmol·L–1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

关键词： cinnamaldehyde     3-hydroxychromone derivative     hydrazine     fluorescent probe    

HTML PDF 收藏

Novel lysosome-targeted anticancer fluorescent agents used in zebrafish and nude mouse tumour imaging

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 112-120 doi: 10.1007/s11705-021-2075-5

摘要： The design of three novel fatty nitrogen mustard-based anticancer agents with fluorophores incorporated into the alkene structure (CXL 118, CXL121, and CXL122) is described in this report. The results indicated that these compounds are selectively located in lysosomes and exhibit effective antitumour activity. Notably, these compounds can directly serve as both reporting and imaging agents in vitro and in vivo without the need to add other fluorescent tagging agents.

关键词： fluorescent drug     lysosomal     anticancer     zebrafish     nude-mouse tumour imaging    

HTML PDF 收藏

Fluorescent probes and functional materials for biomedical applications

《化学科学与工程前沿（英文）》 2022年 第16卷 第10期   页码 1425-1437 doi: 10.1007/s11705-022-2163-1

摘要： Due to their simplicity in preparation, sensitivity and selectivity, fluorescent probes have become the analytical tool of choice in a wide range of research and industrial fields, facilitating the rapid detection of chemical substances of interest as well as the study of important physiological and pathological processes at the cellular level. In addition, many long-wavelength fluorescent probes developed have also proven applicable for in vivo biomedical applications including fluorescence-guided disease diagnosis and theranostics (e.g., fluorogenic prodrugs). Impressive progresses have been made in the development of sensing agents and materials for the detection of ions, organic small molecules, and biomacromolecules including enzymes, DNAs/RNAs, lipids, and carbohydrates that play crucial roles in biological and disease-relevant events. Here, we highlight examples of fluorescent probes and functional materials for biological applications selected from the special issues “Fluorescent Probes” and “Molecular Sensors and Logic Gates” recently published in this journal, offering insights into the future development of powerful fluorescence-based chemical tools for basic biological studies and clinical translation.

关键词： fluorescent probes     imaging     cell     biomedicine     biomolecules    

HTML PDF 收藏

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

《结构与土木工程前沿（英文）》 2019年 第13卷 第6期   页码 1338-1349 doi: 10.1007/s17009-019-0558-2

摘要： In the present study, steel and polypropylene (PP) fibers have been utilized with the intent of obtaining hybrid fiber-reinforced concrete (HFRC) with desirable mechanical properties. An attempt has been made to scrutinize the properties of HFRC with the main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization in producing synergy. Accordingly, a total of 180 specimens, representing 20 different mixtures have been cast and evaluated through compressive, split tensile, and flexural tests. The relevant flexural toughness of the specimens was calculated using ASTM C1018, ASTM C1609, JSCE, and PCS methods, and the effectiveness of these methods was evaluated based on the experimental results. It was observed that steel fibers are more effective in the improvement of flexural toughness in the presence of PP fibers. Furthermore, synergy associated with the combination of fibers at different stages of deflection of the beam specimens was observed and analyzed.

关键词： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

HTML PDF 收藏

标题 作者 时间 类型 操作

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

期刊论文

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

期刊论文

Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products in fish

Yong YU,Laosheng WU

期刊论文

Information gathering and processing with fluorescent molecules

Brian DALY,Jue LING,A. Prasanna de SILVA

期刊论文

基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

期刊论文

海洋鱼类资源的可持续利用和海洋鱼类科学技术的研究方向

林浩然

期刊论文

Recent advances of small-molecule fluorescent probes for detecting biological hydrogen sulfide

期刊论文

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

期刊论文

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

期刊论文

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

期刊论文

Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

期刊论文

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

期刊论文

Novel lysosome-targeted anticancer fluorescent agents used in zebrafish and nude mouse tumour imaging

期刊论文

Fluorescent probes and functional materials for biomedical applications

期刊论文

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

期刊论文